Abstract

Research Article

Expression of Collagen VI, Anticollagenase, Laminin, MM9, Claudins 1 and 5, N and E Cadherins in Choroid Plexus Tumors

Martha Lilia Tena-Suck, Laura Chavez Macias, Erick Gómez-Apo, Alma Ortiz Plata and Carmen Rubio*

Published: 25 November, 2023 | Volume 7 - Issue 1 | Pages: 020-027

Background: CPTs are rare intraventricular papillary neoplasms derived from the choroid plexus epithelium. Anti-collagenase and extracellular matrix which have not been expressed in brain tumors. 
Objective: The purpose of this study was to investigate the expression levels of collagen type VI, anti-collagenase, laminin, MM9, claudins 1 and 5, N and E cadherins, and collagen VII, tejido, and collagen degradation enzyme complexes in choroid plexus tumors.
Materials and methods: We studied the expression of adhesion molecules, extracellular matrix, and anticollagenase with an immunohistochemistry approach and electron microscopy analysis in 42 choroid plexus tumors. 
Results: 28(67%) were choroid plexus papillomas, 8 (19%) were atypical choroid plexus papillomas and 6 (14%) were choroid plexus carcinomas. The Ki67-li and MVD increased from CPC to ACPP, being the highest in malignant tumors as well as a strong immunoexpression of anti-collagenase and were inverse correlation with claudin 5, E, and N cadherin and collagen IV immunoexpressions which added further significant information to the prognosis and varied according to the histologic classification. By ultrastructure, the loss of basal membrane and cilia, disorganization, and proliferation of ECM were observed in CPC. Cerebral homeostasis largely results from the ability of both the Blood–Brain Barrier (BBB) at the brain microvascular endothelium and the Blood–Cerebrospinal Fluid Barrier (BCSFB) at the epithelium of the Choroid Plexuses (CPs), to control the composition of the CSF and cerebral extracellular fluid. Under expression of the tight junction proteins occludin, claudin-1 and claudin-5 are key molecular abnormalities responsible for the increased permeability of tumor endothelial tight junctions. 
Conclusion: The loss of basement membrane and ECM overexpression could be considered as a poor prognosis predictor in CPT. Anti-collagenase and MMP9 overexpression could be related to basal membrane and BBB plasticity in CPTs.

Read Full Article HTML DOI: 10.29328/journal.apcr.1001037 Cite this Article Read Full Article PDF

Keywords:

Choroid plexus; Choroid plexus tumors; Extracellular matrix; Collagen IV; Claudins 1 and 5; Immunohistochemistry

References

  1. Ruggeri L, Alberio N, Alessandrello R, Cinquemani G, Gambadoro C, Lipani R, Maugeri R, Nobile F, Iacopino DG, Urrico G, Battaglia R. Rapid malignant progression of an intraparenchymal choroid plexus papillomas. Surg Neurol Int. 2018 Jul 5;9:131. doi: 10.4103/sni.sni_434_17. PMID: 30105129; PMCID: PMC6044141.
  2. Spennato P, De Martino L, Russo C, Errico ME, Imperato A, Mazio F, Miccoli G, Quaglietta L, Abate M, Covelli E, Donofrio V, Cinalli G. Tumors of Choroid Plexus and Other Ventricular Tumors. Adv Exp Med Biol. 2023;1405:175-223. doi: 10.1007/978-3-031-23705-8_7. PMID: 37452939.
  3. Mihaljevic S, Michalicova A, Bhide M, Kovac A. Pathophysiology of the choroid plexus in brain diseases. Gen Physiol Biophys. 2021 Nov;40(6):443-462. doi: 10.4149/gpb_2021032. PMID: 34897020.
  4. Thomas C, Soschinski P, Zwaig M, Oikonomopoulos S, Okonechnikov K, Pajtler KW, Sill M, Schweizer L, Koch A, Neumann J, Schüller U, Sahm F, Rauschenbach L, Keyvani K, Proescholdt M, Riemenschneider MJ, Segewiß J, Ruckert C, Grauer O, Monoranu CM, Lamszus K, Patrizi A, Kordes U, Siebert R, Kool M, Ragoussis J, Foulkes WD, Paulus W, Rivera B, Hasselblatt M. The genetic landscape of choroid plexus tumors in children and adults. Neuro Oncol. 2021 Apr 12;23(4):650-660. doi: 10.1093/neuonc/noaa267. PMID: 33249490; PMCID: PMC8041331.
  5. Adib SD, Hempel JM, Kandilaris K, Grimm F, Zamora RE, Tatagiba M. Surgical management of choroid plexus papilloma of the cerebellopontine and cerebellomedullary angle: classification and strategy. Neurosurg Rev. 2021 Dec;44(6):3387-3397. doi: 10.1007/s10143-021-01506-4. Epub 2021 Feb 24. Erratum in: Neurosurg Rev. 2021 Apr 12;: PMID: 33629235; PMCID: PMC8592964.
  6. Karamanos NK, Theocharis AD, Piperigkou Z, Manou D, Passi A, Skandalis SS, Vynios DH, Orian-Rousseau V, Ricard-Blum S, Schmelzer CEH, Duca L, Durbeej M, Afratis NA, Troeberg L, Franchi M, Masola V, Onisto M. A guide to the composition and functions of the extracellular matrix. FEBS J. 2021 Dec;288(24):6850-6912. doi: 10.1111/febs.15776. Epub 2021 Mar 23. PMID: 33605520.
  7. Wang W, Li S, Wang X, Wang J, Zhang Y. PbO nanoparticles increase the expression of ICAM-1 and VCAM-1 by increasing reactive oxygen species production in choroid plexus. Environ Sci Pollut Res Int. 2023 Mar;30(14):40162-40173. doi: 10.1007/s11356-022-25109-8. Epub 2023 Jan 6. PMID: 36607576.
  8. Nyimanu D, Behm C, Choudhury S, Yu ASL. The role of claudin-2 in kidney function and dysfunction. Biochem Soc Trans. 2023 Aug 31;51(4):1437-1445. doi: 10.1042/BST20220639. PMID: 37387353.
  9. Berndt P, Winkler L, Cording J, Breitkreuz-Korff O, Rex A, Dithmer S, Rausch V, Blasig R, Richter M, Sporbert A, Wolburg H, Blasig IE, Haseloff RF. Tight junction proteins at the blood-brain barrier: far more than claudin-5. Cell Mol Life Sci. 2019 May;76(10):1987-2002. doi: 10.1007/s00018-019-03030-7. Epub 2019 Feb 7. PMID: 30734065.
  10. Raya-Sandino A, Lozada-Soto KM, Rajagopal N, Garcia-Hernandez V, Luissint AC, Brazil JC, Cui G, Koval M, Parkos CA, Nangia S, Nusrat A. Claudin-23 reshapes epithelial tight junction architecture to regulate barrier function. Nat Commun. 2023 Oct 5;14(1):6214. doi: 10.1038/s41467-023-41999-9. PMID: 37798277; PMCID: PMC10556055.
  11. Arapatzi C, Rouni G, Kostourou V. Vascular cell-matrix adhesion in development and cancer. Int J Dev Biol. 2022;66(1-2-3):103-113. doi: 10.1387/ijdb.210204vk. PMID: 34881799.
  12. Taddei E, Rosiles A, Hernandez L, Luna R, Rubio C. Apoptosis in the Dentate Nucleus Following Kindling-induced Seizures in Rats. CNS Neurol Disord Drug Targets. 2022;21(6):511-519. doi: 10.2174/1871527320666211201161800. PMID: 34852754.
  13. Dani N, Herbst RH, McCabe C, Green GS, Kaiser K, Head JP, Cui J, Shipley FB, Jang A, Dionne D, Nguyen L, Rodman C, Riesenfeld SJ, Prochazka J, Prochazkova M, Sedlacek R, Zhang F, Bryja V, Rozenblatt-Rosen O, Habib N, Regev A, Lehtinen MK. A cellular and spatial map of the choroid plexus across brain ventricles and ages. Cell. 2021 May 27;184(11):3056-3074.e21. doi: 10.1016/j.cell.2021.04.003. Epub 2021 Apr 30. PMID: 33932339; PMCID: PMC8214809.
  14. Moon CS, Moon D, Kang SK. Aquaporins in Cancer Biology. Front Oncol. 2022 Jun 29;12:782829. doi: 10.3389/fonc.2022.782829. PMID: 35847914; PMCID: PMC9278817.
  15. Kadry H, Noorani B, Cucullo L. A blood-brain barrier overview on structure, function, impairment, and biomarkers of integrity. Fluids Barriers CNS. 2020 Nov 18;17(1):69. doi: 10.1186/s12987-020-00230-3. PMID: 33208141; PMCID: PMC7672931.
  16. Kakogiannos N, Ferrari L, Giampietro C, Scalise AA, Maderna C, Ravà M, Taddei A, Lampugnani MG, Pisati F, Malinverno M, Martini E, Costa I, Lupia M, Cavallaro U, Beznoussenko GV, Mironov AA, Fernandes B, Rudini N, Dejana E, Giannotta M. JAM-A Acts via C/EBP-α to Promote Claudin-5 Expression and Enhance Endothelial Barrier Function. Circ Res. 2020 Sep 25;127(8):1056-1073. doi: 10.1161/CIRCRESAHA.120.316742. Epub 2020 Jul 15. PMID: 32673519; PMCID: PMC7508279.
  17. Ohta Y, Fujii M, Takahashi S, Takano A, Nanki K, Matano M, Hanyu H, Saito M, Shimokawa M, Nishikori S, Hatano Y, Ishii R, Sawada K, Machinaga A, Ikeda W, Imamura T, Sato T. Cell-matrix interface regulates dormancy in human colon cancer stem cells. Nature. 2022 Aug;608(7924):784-794. doi: 10.1038/s41586-022-05043-y. Epub 2022 Jul 7. PMID: 35798028.
  18. Miller AD, Miller CR, Rossmeisl JH. Canine Primary Intracranial Cancer: A Clinicopathologic and Comparative Review of Glioma, Meningioma, and Choroid Plexus Tumors. Front Oncol. 2019 Nov 8;9:1151. doi: 10.3389/fonc.2019.01151. PMID: 31788444; PMCID: PMC6856054.
  19. Muscatello LV, Avallone G, Serra F, Seuberlich T, Mandara MT, Sisó S, Brunetti B, Oevermann A. Glomeruloid Microvascular Proliferation, Desmoplasia, and High Proliferative Index as Potential Indicators of High Grade Canine Choroid Plexus Tumors. Vet Pathol. 2018 May;55(3):391-401. doi: 10.1177/0300985817754124. Epub 2018 Feb 5. PMID: 29402204.
  20. Gloushankova NA, Zhitnyak IY, Rubtsova SN. Role of Epithelial-Mesenchymal Transition in Tumor Progression. Biochemistry (Mosc). 2018 Dec;83(12):1469-1476. doi: 10.1134/S0006297918120052. PMID: 30878022.
  21. LaFoya B, Munroe JA, Miyamoto A, Detweiler MA, Crow JJ, Gazdik T, Albig AR. Beyond the Matrix: The Many Non-ECM Ligands for Integrins. Int J Mol Sci. 2018 Feb 2;19(2):449. doi: 10.3390/ijms19020449. PMID: 29393909; PMCID: PMC5855671.
  22. Eble JA, Niland S. The extracellular matrix in tumor progression and metastasis. Clin Exp Metastasis. 2019 Jun;36(3):171-198. doi: 10.1007/s10585-019-09966-1. Epub 2019 Apr 11. PMID: 30972526.
  23. Hinneh JA, Gillis JL, Mah CY, Irani S, Shrestha RK, Ryan NK, Atsushi E, Nassar ZD, Lynn DJ, Selth LA, Kato M, Centenera MM, Butler LM. Targeting hyaluronan-mediated motility receptor (HMMR) enhances response to androgen receptor signalling inhibitors in prostate cancer. Br J Cancer. 2023 Oct;129(8):1350-1361. doi: 10.1038/s41416-023-02406-8. Epub 2023 Sep 6. PMID: 37673961; PMCID: PMC10575850.
  24. Ramos-Lewis W, LaFever KS, Page-McCaw A. A scar-like lesion is apparent in basement membrane after wound repair in vivo. Matrix Biol. 2018 Dec;74:101-120. doi: 10.1016/j.matbio.2018.07.004. Epub 2018 Jul 5. PMID: 29981372; PMCID: PMC6250587.
  25. Ramos-Lewis W, Page-McCaw A. Basement membrane mechanics shape development: Lessons from the fly. Matrix Biol. 2019 Jan;75-76:72-81. doi: 10.1016/j.matbio.2018.04.004. Epub 2018 Apr 12. PMID: 29656148; PMCID: PMC6185827.
  26. Wakamatsu K, Chiba Y, Murakami R, Matsumoto K, Miyai Y, Kawauchi M, Yanase K, Uemura N, Ueno M. Immunohistochemical expression of osteopontin and collagens in choroid plexus of human brains. Neuropathology. 2022 Apr;42(2):117-125. doi: 10.1111/neup.12791. Epub 2021 Dec 28. PMID: 34964160; PMCID: PMC9546339.
  27. Maeba T, Yonezawa T, Ono M, Tomono Y, Heljasvaara R, Pihlajaniemi T, Inagawa K, Oohashi T. Collagen XVIII Deposition in the Basement Membrane Zone beneath the Newly Forming Epidermis during Wound Healing in Mice. Acta Med Okayama. 2019 Apr;73(2):135-146. doi: 10.18926/AMO/56649. PMID: 31015748.

Figures:

Figure 1

Figure 1

Figure 1

Figure 2

Figure 1

Figure 3

Figure 1

Figure 4

Figure 1

Figure 5

Figure 1

Figure 6

Similar Articles

Recently Viewed

Read More

Most Viewed

Read More

Help ?